m hominis reference strain pg21  (ATCC)

Journal: Frontiers in Microbiology

Article Title: A genome-wide investigation of Mycoplasma hominis genes associated with gynecological infections or infertility

doi: 10.3389/fmicb.2025.1561378

Figure Lengend Snippet: COG classification of genes. (A) Clusters of orthologous genes (COGs) distribution among the 52 M. hominis strains associated with infertility. (B) COGs distribution among the 10 M. hominis strains associated with genital infections. Bars in green, red, and blue, represent the core genome, the accessory genes (shell genes), and unique genes (cloud genes), respectively.

Article Snippet: The genome size of M. hominis reference strain PG21 (ATCC 23114) was estimated to be around 665 kb (Calcutt and Foecking, ), thus exceeding Mycoplasma genitalium genome (580 kb), the smallest genome of any organism that can be grown in pure culture (Fookes et al., ), by only 85 kb.

Techniques:

Journal: Frontiers in Microbiology

Article Title: A genome-wide investigation of Mycoplasma hominis genes associated with gynecological infections or infertility

doi: 10.3389/fmicb.2025.1561378

Figure Lengend Snippet: Single nucleotide polymorphism (SNP) distribution among M. hominis strains associated with gynecological infections (red boxplot) and infertility (blue boxplot). Student's t -test was used to assess for statistical significant difference ( P < 0.05) between the mean SNPs count values of strains linked to infertility and gynecological infections. * Represents the mean SNP count of each boxplot.

Article Snippet: The genome size of M. hominis reference strain PG21 (ATCC 23114) was estimated to be around 665 kb (Calcutt and Foecking, ), thus exceeding Mycoplasma genitalium genome (580 kb), the smallest genome of any organism that can be grown in pure culture (Fookes et al., ), by only 85 kb.

Techniques:

Journal: Frontiers in Microbiology

Article Title: A genome-wide investigation of Mycoplasma hominis genes associated with gynecological infections or infertility

doi: 10.3389/fmicb.2025.1561378

Figure Lengend Snippet: TreeWAS-based phylogenetic tree illustrating the relatedness between the 62 Tunisian M. hominis clinical strains based on pathotype association. Strains in red are associated with gynecological infections pathotype and strains in blue are associated with infertility pathotype.

Article Snippet: The genome size of M. hominis reference strain PG21 (ATCC 23114) was estimated to be around 665 kb (Calcutt and Foecking, ), thus exceeding Mycoplasma genitalium genome (580 kb), the smallest genome of any organism that can be grown in pure culture (Fookes et al., ), by only 85 kb.

Techniques:

Journal: Scientific Reports

Article Title: Random transposon insertion in the Mycoplasma hominis minimal genome

doi: 10.1038/s41598-019-49919-y

Figure Lengend Snippet: In silico analyses of restriction-modification systems in the sequenced M . hominis .

Article Snippet: The M . hominis reference strains PG21 (ATCC 23114) and M132 (ATCC 43521) and the clinical isolates 2674, 3299, 331, 3364, 35, 3631, 4016, 4235, 4788, 4796, 5012, 5060, and 5096 (SRA accession number: PRJNA493181) were grown at 37 °C in Hayflick modified medium supplemented with arginine for 24 to 48 hours .

Techniques: In Silico

Journal: Scientific Reports

Article Title: Random transposon insertion in the Mycoplasma hominis minimal genome

doi: 10.1038/s41598-019-49919-y

Figure Lengend Snippet: Variable parameters during M . hominis transformation assays. All of the different conditions assayed during the development of the transformation protocol are detailed in rectangles.

Article Snippet: The M . hominis reference strains PG21 (ATCC 23114) and M132 (ATCC 43521) and the clinical isolates 2674, 3299, 331, 3364, 35, 3631, 4016, 4235, 4788, 4796, 5012, 5060, and 5096 (SRA accession number: PRJNA493181) were grown at 37 °C in Hayflick modified medium supplemented with arginine for 24 to 48 hours .

Techniques: Transformation Assay

Journal: Scientific Reports

Article Title: Random transposon insertion in the Mycoplasma hominis minimal genome

doi: 10.1038/s41598-019-49919-y

Figure Lengend Snippet: Successful transformation parameters and transformation efficiencies (in number of transformants/CFU/µg of plasmid) for M . hominis PEG-mediated transformation.

Article Snippet: The M . hominis reference strains PG21 (ATCC 23114) and M132 (ATCC 43521) and the clinical isolates 2674, 3299, 331, 3364, 35, 3631, 4016, 4235, 4788, 4796, 5012, 5060, and 5096 (SRA accession number: PRJNA493181) were grown at 37 °C in Hayflick modified medium supplemented with arginine for 24 to 48 hours .

Techniques: Transformation Assay, Plasmid Preparation, Concentration Assay

Journal: Scientific Reports

Article Title: Random transposon insertion in the Mycoplasma hominis minimal genome

doi: 10.1038/s41598-019-49919-y

Figure Lengend Snippet: Position of the tet (M) gene in the M . hominis M132 transformants.

Article Snippet: The M . hominis reference strains PG21 (ATCC 23114) and M132 (ATCC 43521) and the clinical isolates 2674, 3299, 331, 3364, 35, 3631, 4016, 4235, 4788, 4796, 5012, 5060, and 5096 (SRA accession number: PRJNA493181) were grown at 37 °C in Hayflick modified medium supplemented with arginine for 24 to 48 hours .

Techniques:

Journal: Scientific Reports

Article Title: Random transposon insertion in the Mycoplasma hominis minimal genome

doi: 10.1038/s41598-019-49919-y

Figure Lengend Snippet: Insertion site of the tet (M) gene in the genome of the M . hominis M132 transformant 28-2. ( A ) Scheme of the transposon inserted into the M . hominis M132 genome. The region contains inverted repeats (point rectangles), the sequence of the pMT85 plasmid (white rectangles) and the tet (M) gene (dark gray rectangle). ( B ) Scheme of insertion site for transformant 28-2. Hatched rectangles represent the M . hominis M132 gene where the tet (M) gene inserted. Double thin lines represent the position of the insertion inside the M . hominis M132 gene. Clear gray rectangles correspond to sequence of the bacterial genome. Numbers above single thin lines indicate the position around the genome. Black arrows indicate the position of the PCR primers P75-F1 and P75-R1, and gray arrows indicate the position of the PCR primers P75-F2 and P75-R2.

Article Snippet: The M . hominis reference strains PG21 (ATCC 23114) and M132 (ATCC 43521) and the clinical isolates 2674, 3299, 331, 3364, 35, 3631, 4016, 4235, 4788, 4796, 5012, 5060, and 5096 (SRA accession number: PRJNA493181) were grown at 37 °C in Hayflick modified medium supplemented with arginine for 24 to 48 hours .

Techniques: Sequencing, Plasmid Preparation

Journal: Scientific Reports

Article Title: Random transposon insertion in the Mycoplasma hominis minimal genome

doi: 10.1038/s41598-019-49919-y

Figure Lengend Snippet: Test of adhesion to HeLa cells. The graph shows the quantity of adhered M . hominis (copies/µL) as a function of the M . hominis inoculum (in CFU/mL). Blue points correspond to the M132 wild-type strain and gray points correspond to the 28-2 mutant. This experiment was performed in triplicate, and average values are represented. Standard deviation represents the above points by vertical lines.

Article Snippet: The M . hominis reference strains PG21 (ATCC 23114) and M132 (ATCC 43521) and the clinical isolates 2674, 3299, 331, 3364, 35, 3631, 4016, 4235, 4788, 4796, 5012, 5060, and 5096 (SRA accession number: PRJNA493181) were grown at 37 °C in Hayflick modified medium supplemented with arginine for 24 to 48 hours .

Techniques: Mutagenesis, Standard Deviation

Journal: BMC Genomics

Article Title: Generation of Mycoplasma hominis gene-targeted mutants by targeting-induced local lesions in genomes (TILLING)

doi: 10.1186/s12864-018-4917-1

Figure Lengend Snippet: Effects of EMS mutagenesis on M. hominis survival. Concentrations of M. hominis PG21 were determined after treatment with various concentration of EMS for 1.5 h and 3 h ( a ). Three independent experiments were performed. The percentage of killing was calculated ( b ). In accordance with the literature, a 75% killing rate corresponding to exposure to approximately 9 mg/ml EMS for 3 h was chosen to generate the M. hominis PG21 mutant library

Article Snippet: The M. hominis PG21 reference strain was from ATCC (ATCC 23114).

Techniques: Mutagenesis, Concentration Assay

Journal: BMC Genomics

Article Title: Generation of Mycoplasma hominis gene-targeted mutants by targeting-induced local lesions in genomes (TILLING)

doi: 10.1186/s12864-018-4917-1

Figure Lengend Snippet: vaa - and oppA -mutants obtained by screening the 1200 EMS-generated M. hominis clone library

Article Snippet: The M. hominis PG21 reference strain was from ATCC (ATCC 23114).

Techniques: Mutagenesis

Journal: BMC Genomics

Article Title: Generation of Mycoplasma hominis gene-targeted mutants by targeting-induced local lesions in genomes (TILLING)

doi: 10.1186/s12864-018-4917-1

Figure Lengend Snippet: Mutations harboured by EMS-generated M. hominis mutants in comparison to the sequence of the M. hominis PG21 strain not exposed to EMS

Article Snippet: The M. hominis PG21 reference strain was from ATCC (ATCC 23114).

Techniques: Comparison, Sequencing, Mutagenesis

Journal: BMC Genomics

Article Title: Generation of Mycoplasma hominis gene-targeted mutants by targeting-induced local lesions in genomes (TILLING)

doi: 10.1186/s12864-018-4917-1

Figure Lengend Snippet: Adhesion to HeLa cells of EMS-generated M. hominis vaa -mutants. M. hominis cells were added to immobilized HeLa cells in triplicate and incubated 4 h at 37 °C. After removal of unbound M. hominis cells by washing, the adherent M. hominis cells were quantified by real-time PCR. One representative experiment of three is shown

Article Snippet: The M. hominis PG21 reference strain was from ATCC (ATCC 23114).

Techniques: Generated, Incubation, Real-time Polymerase Chain Reaction

Journal: BMC Genomics

Article Title: Generation of Mycoplasma hominis gene-targeted mutants by targeting-induced local lesions in genomes (TILLING)

doi: 10.1186/s12864-018-4917-1

Figure Lengend Snippet: ecto ATPase activity of EMS-generated M. hominis oppA -mutants. ecto ATPase activity of EMS-generated Mho369 and Mho940 M. hominis mutants and of M. hominis PG21 parental strain was determined by measuring the release of free phosphate per 10 10 cells as a function of the ATP concentration. The data represent the means and standard errors of five (Mho369) and four (Mho940 and PG21) independent experiments with triplicate samples in each experiment. V max , maximum rate, K m , Michaelis-Menten constant, 95% IC, 95% confidence interval

Article Snippet: The M. hominis PG21 reference strain was from ATCC (ATCC 23114).

Techniques: Activity Assay, Generated, Concentration Assay